Friday, December 15, 2017

Gopalasamudram Narayanan Ramachandran, Biological Physicist

Many followers of the Intermediate Physics for Medicine and Biology Facebook page are from India, and I would like to somehow thank them for their interest in our book. The only way I can express my appreciation is by writing in this blog. So, today’s post is about the great Indian physicist Gopalasamudram Narayanan Ramachandran (1922-2001).

In an obituary published in the Biographical Memoirs of Fellows of the Royal Society (Volume 51, Pages 367-377, 2005), Vijayan and Johnson write
G. N. Ramachandran has been among the most outstanding crystallographers and structural biologists of our times. He is considered by many to be the best scientist to have worked in independent India. The model of collagen developed by him has stood the test of time and has contributed greatly to understanding the role of this important fibrous protein. His pioneering contributions in crystallography, particularly in relation to methods of structure analysis using Fourier techniques and anomalous dispersion, are well recognized. A somewhat less widely recognized contribution of his is concerned with three-dimensional image reconstruction. Much of the foundation of the currently thriving field of molecular modelling was laid by him. The Ramachandran plot remains the simplest and the most commonly used descriptor and tool for the validation of protein structures.”
Ramachandran appears in IPMB in Chapter 12, when Russ Hobbie and I discuss computed tomography. He and A. V. Lakshminarayanan developed one of the two man main tomographic techniques: filtered back projection. We write
“Filtered back projection is more difficult to understand than the direct Fourier technique. It is easy to see that every point in the object contributes to some point in each projection. The converse is also true. In a back projection every point in each projection contributes to some point in the reconstructed image…A very simple procedure would be to construct an image by back-projecting every projection…We will now show that the image fb(x,y) obtained by taking projections of the object F(θ,x’) and then backprojecting them is equivalent to taking the convolution of the object with the function h.”
h(x) is
Unfortunately, this function does not exist; the integral doesn’t converge. The factor |k| diverges as k goes to ±∞. But Ramachandran and Lakshminarayanan realized that you don’t need to actually integrate to infinity. In the above integral, k is the spatial frequency. He suggested there should be an upper limit on the spatial frequency, kmax. What should the upper limit be? The measured projection F(θ,x’) is not a continuous function of position x’. The data is discrete, measured at a finite number of points. The largest spatial frequency is that given by the Nyquist sampling criterion: there should be at least two points per wavelength. Using this upper limit for kmax, Ramachandran and Lakshminarayanan were able to solve the integral for h(x) analytically, and found that
where i denotes the ith discrete value of x. This result looks slightly different than Eq. 12.44 in IPMB: Here I factored N2/16 out of each term, and I use i for the integer instead of k, because I don’t want to use k for both spatial frequency and an integer. Below is a plot of h(i).
Convolution with function h(i) corresponds to a passing the signal through a high pass filter (often called the "Ram-Lak filter"). Therefore, the convolution of a constant should vanish, implying that all the values of h(i) should add to zero. In fact, this is true. The infinite series
 is exactly what is needed to ensure this.

At the end of their obituary, Vijayan and Johnson discuss Ramachandran's impact on science and India.
“To more than a generation of scientists in India, and some abroad, Ramachandran was a source of scientific and personal inspiration. Many of his contributions were based on simple but striking ideas. He demonstrated how international science could be influenced, even from less well-endowed neighbourhoods, through ingenuity and imagination. It is remarkable that although Ramachandran left structural biology and mainstream research about a quarter of century ago, his presence in the field remains as vibrant as ever. Indeed, Ramachandran established a great scientific tradition. That tradition lives on and thrives in the world, in India, and in the two research schools he founded.”
Thanks to all the Indian readers of IPMB. I'm glad you like the book.

Friday, December 8, 2017

Shattered Nerves: How Science is Solving Modern Medicine's Most Perplexing Problem

In his book Shattered Nerves: How Science is Solving Modern Medicine’s Most Perplexing Problem, Victor Chase tells the story of neural prostheses. Russ Hobbie and I discuss neural stimulation in Section 7.10 of Intermediate Physics for Medicine and Biology.
“The information that has been developed in this chapter can also be used to understand some of the features of stimulating electrodes. These may be used for electromyographic studies; for stimulating muscle to contract called functional electrical stimulation (Peckham and Knutson 2005); for a cochlear implant to partially restore hearing (Zeng et al. 2008); deep brain stimulation for Parkinson’s disease (Perlmutter and Mink 2006); for cardiac pacing (Moses and Mullin 2007); and even for defibrillation (Dosdall et al. 2009).”
Chase begins by describing the cochlear implant. A common cause of deafness is the death of hair cells in the cochlea, leaving the auditory nerve intact but not activated by sound. A cochlear implant stimulates the auditory nerve using several electrodes, each corresponding to a different frequency. Chase often describes medical devices from the point of view of a patient, and in this case he tells the story of Michael Pierschalla, who not only benefited from this technology but contributed to its development.

I am fascinated by idiosyncratic inventors such as Giles Brindley. Chase writes
“An often-told tale about Giles Brindley might reveal something about the person referred to as the grandfather of neural prostheses. In 1983, the inveterate innovator and self-experimenter stood before a scientific audience and removed his pants. The venue was Las Vegas, Nevada, and the audience that witnessed this occurrence was the membership of the American Urological Association. Brindley was demonstrating, quite graphically, the success of an injection of phenoxybenzamine, a treatment he had developed for erectile dysfunction.”
Brindley developed one of the first visual prostheses that stimulated the brain. He also invented a musical instrument he called the "undilector," which is something like a computer-controlled bassoon.

One hero of Chase’s story is F. Terry Hambrecht, who led the National Institutes of Health Neural Prosthesis Program. When I was working at the NIH intramural program in the early 1990s, I often attended Hambrecht’s annual Neural Prosthesis Workshop. Sometimes I would submit a poster about magnetic stimulation. It was close enough to the workshop’s theme to be worth a poster, but far enough from its main thrust to be a little off-topic. At these workshops, held on the NIH campus, I met many of the scientists highlighted by Chase.

Shattered Nerves focuses on research performed at Case Western Reserve University. J. Thomas Mortimer founded the Applied Neural Control Laboratory there. His student P. Hunter Peckham developed a prosthetic device to restore function to a patient's paralyzed hand. Another Case researcher, Ronald Triolo, invented a stimulator that allowed a wheelchair-bound patient to stand and move around. Quadriplegics often have difficulty controlling their urination and bowel movements. Mortimer and Graham Creasey developed a prosthesis to control the bladder and bowel muscles.

Rather than summarizing Shattered Nerves myself, I will let Chase do so in his own words.
"Unfortunately, in some people, the circuitry that generates and conducts electrical signals goes bad, rendering them unable to fully partake of the miracle of the senses, as in the case of the blind, when the rod and cone photoreceptors inside the eye can no longer translate light into the electrical signals that send information to the brain. Or when the hair cells inside the cochlea of the inner ear, which process sound waves, die off, and a person loses the ability to hear. Failure of the body's electrical circuitry is also responsible for paralysis that occurs when spinal cord injuries damage the nerve cells that carry electrical signals from the brain's motor cortex to the muscles and from the skin's tactile receptors to the somatosensory portion of the brain. Until recently, these conditions were deemed irreversible. Now there is hope."
What did I gain from reading Shattered Nerves? First, I like to study the history of a field in order to better appreciate the current problems and future directions. Second, the researchers and patients that Chase describes are inspirational. Third, I was amazed at how these pioneers combined physics and engineering with medicine and biology, as Russ and I advocate in IPMB.

All books have advantages and disadvantages. One disadvantage is that Shattered Nerves was written in 2006. In a fast moving field like neural prostheses, I wish the book was up-to-date. An advantage is that you can read it for free through Project Muse.

Enjoy!

Friday, December 1, 2017

Suki Has Fleas

Suki has fleas. It is her worst infestation ever. My wife and I have battled them for about a month, and are finally gaining the upper hand by constantly vacuuming the house, washing her bedding, and giving her baths.

While I am sure you empathize with our little puppy, you are probably asking “what do Suki's fleas have to do with Intermediate Physics for Medicine and Biology?” A lot! In Problem 47 of Chapter 2, Russ Hobbie and I ask students to determine how jumping height scales with mass. I won’t give away the answer here, but let me note that when you are asked how something scales with mass, one possible answer is that it doesn’t. In other words, if the allometric relationship is Jumping Height = C Massn, where C and n are constants, then one possible value for n is zero; jumping height is independent of mass.

The next homework exercise, Problem 48, analyzes one of the many scaling arguments made by Knut Schmidt-Nielsen in his marvelous book Scaling: Why is Animal Size so Important? The start of Problem 48 gives away the answer to Problem 47:
Problem 48. In Problem 47, you should have found that all animals can jump to about the same height (approximately 0.6 m), independent of their mass M.”
Are you skeptical that, for instance, a tiny flea can jump 60 cm (about two feet)? I can tell you from first-hand experience that those little buggers can really jump. Each evening we inspect Suki with a flea comb, and sometimes a flea jumps away before we can kill it. 

Problem 48 requires that students calculate the flea's acceleration. Again, I won't give you the answer, but those fleas sure undergo large accelerations! If you don’t believe me, do Problem 48, or read what Knut Schmidt-Nielsen writes:
"For a flea, acceleration takes place over less than 1 mm, and takeoff time is less than 1 msec. The average acceleration during takeoff must therefore exceed 200 g. It is worth a moment's reflection to think of what such high acceleration means. It means that the force on the animal is 200 times its weight (any mammal would be totally crushed under such forces), and the insect must have a skeleton and internal organs able to resist such acceleration forces."
I wonder how fleas avoid concussions?

I’m glad that our little fleabag is cleaning up her act. Suki turned 15 a few months ago, and she is the old lady of the family. But she is still up for our walks, during which I listen to audiobooks and she snoots around (that’s probably how she got the fleas). And what is her favorite book? Intermediate Physics for Medicine and Biology, of course.

Friday, November 24, 2017

Top Ten Journal Articles Cited in IPMB

Russ Hobbie and I cite many articles in Intermediate Physics for Medicine and Biology. In the introduction to an early edition of IPMB, Russ wrote:
“I also hope that research workers in biology and medicine will find it a useful reference to brush up on the physics they need or to find a few pointers to the current literature in a number of areas of biophysics. (The bibliography in each chapter is by no means exhaustive; however, the references should lead you quickly into a field.)”
Below is a list of my favorite dozen articles cited in IPMB. I am only considering those published in journals; books and book chapters are another story. This is not a list of the most important papers, nor the best written papers, nor the most cited papers. These are my personal favorites. Let's count'em down to number one!


12. Barker AT, Jalinous R, Freeston IL (1985) Non-invasive magnetic stimulation of the human cortex. Lancet 1(8437):1106–1107
This paper founded the field of transcranial magnetic stimulation, which is a topic I worked on when at the National Institutes of Health in the 1990s. See here for more about Tony Barker. The Lancet is one of England’s top medical journals; think of it as a British version of The New England Journal of Medicine.
11. Lubin JH (1999) Response to Cohen’s comments on the Lubin rejoinder. Health Phys 77(3):330–332
Russ and I cite a back-and-forth series of letters and replies by Bernard Cohen and Jay Lubin regarding the health effects of low levels of radiation. Although these authors address a serious issue and each has a strong opinion, my impression is that they had a little fun with this exchange, at least when making up the titles. Health Physics is the official journal of the Health Physics Society.
10. Mermin ND (1994) Stirling’s formula! Am J Phys 52: 362–365.
I am cheating a little bit with this paper. It wasn’t cited in one of the list of references found at the end of each chapter in IPMB, but rather in a footnote of Appendix I. I included it because David Mermin is my favorite writer of physics, and I couldn’t bear to leave him off the list. Also, the article appeared in my favorite journal, the American Journal of Physics. Finally, the title contains an exclamation point (a subtle pun)!
9. Foster KR, Moulder JE (2013) Wi-Fi and health: review of current status and research. Health Phys 105(6):561–575
Ken Foster and John Moulder have been fighting the good fight for years, debunking pseudoscience about the biological effects of weak electric and magnetic fields. This 2013 paper, published in Health Physics, honors their body of work. We need more from these two.
8. West GB, Brown JH, Enquist BJ (1999) The fourth dimension of life: fractal geometry and allometric scaling of organisms. Science 284:1677–1679.
One of my favorite chapters in IPMB, Chapter 2, is about the exponential function. It discusses Kleiber’s law: metabolic rate scales as mass to the ¾ power. Why ¾? This is a long-standing and fundamental question in biology. Geoffrey West and his collaborators offer a possible explanation. The paper was published in Science, the academic journal of the American Association for the Advancement of Science. It is perhaps the most famous scientific journal.
7. Glide-Hurst CK, Maidment ADA, Orton CG (2010) Point/counterpoint: Ultrasonography is soon likely to become a viable alternative to x-ray mammography for breast cancer screening. Med Phys 37:4526–4529
The journal Medical Physics publishes a Point/Counterpoint article ever month, where prominent medical physicists debate a controversial issue. They are fun to read and a great teaching tool. Carri Glide-Hurst is a medical physicist working at Henry Ford Hospital in Detroit and is the mentor for an Oakland University graduate student, so she gets points for being part of the home team.
6. Hämäläinen M, Hari R, Ilmoniemi RJ, Knuutila J, Lounasmaa OV (1993) Magnetoencephalography—theory, instrumentation, and applications to noninvasive studies of the working human brain. Rev Mod Phys 65(2):413–497
This review article on magnetoencephography by a group of Finnish scientists is so good that I still refer students to it even though it is nearly 25 years old.
5. Clark J, Plonsey R (1968) The extracellular potential field of a single active nerve fiber in a volume conductor. Biophys J 8:842–864
John Clark and Robert Plonsey’s paper on the extracellular potential of a nerve axon influenced my work as a graduate student. Plonsey was a giant in the field of bioelectricity, Clark was his graduate student, and the Biophysical Journal is one of the top scientific journals publishing papers at the intersection of physics and biology.
4. Hobbie RK (1973) The electrocardiogram as an example of electrostatics. Am J Phys 41:824–831
This is one of several articles Russ published about medical and biological physics, which ultimately led to his writing IPMB.
3. Basser PJ, Mattiello J, LeBihan D (1994) MR diffusion tensor spectroscopy and imaging. Biophys J 66:259–267
In this paper Peter Basser and his coworkers invented MRI Diffusion Tensor Imaging. Peter's office was just down the hall from mine when we both worked at the National Institutes of Health, and I can remember the morning he brought in a chunk of pork to do the experiments described in this article. I also knew James Mattiello at NIH; he was one of the first graduates of the Oakland University Medical Physics PhD Program.
2. Hodgkin AL, Huxley AF (1952) A quantitative description of membrane current and its application to conduction and excitation in nerve. J Physiol 117:500–544
Every semester I give this classic paper to the students in my Biological Physics class, and we go through it figure by figure when studying Chapter 6 in IPMB. It is truly a Nobel Prize quality paper.
1. Purcell EM (1977) Life at low Reynolds number. Am J Phys 45:3–11
This article is a gem: Wonderfully written by a Nobel Prize winner, charming hand-drawn figures, and published in my favorite journal.

Friday, November 17, 2017

William Bialek, Winner of the 2018 Max Delbruck Prize in Biological Physcis

William Bialek is this year’s winner of the American Physical Society’s 2018 Max Delbruck Prize in Biological Physics “for the application of general theoretical principles of physics and information theory to help understand and predict how biological systems function across a variety of scales, form molecules and cells, to brains and animal collectives.”

Bialek is author of the textbook Biophysics: Searching for Principles. When preparing this blog post, I checked out this book from the Oakland University Library and glanced through it. It is different from Intermediate Physics for Medicine and Biology in many ways. For instance, it is a graduate text, aimed at grad students in physics, whereas IPMB is targeted at undergraduates who have had a year of introductory physics and a year of calculus. Biophysics emphasizes events at the molecular scale, while in the preface to IPMB Russ Hobbie and I write "molecular biophysics has been almost completely ignored: excellent texts already exist, and this is not our area of expertise." One of those excellent texts would be Bialek's Biophysics.

I particularly enjoyed Bialek's introduction, which is a readable and personal account of his experiences at the intersection of physics and biology. At one point he lists a series of questions that anyone interested in applying physics to biology should think about:
  • "Where is the boundary between physics and biology?
  • Is biophysics really physics or just the application of methods from physics to the problems of biology?
  • My biologist friends tell me that 'theoretical biology' is nonsense [Yikes!], so what would theoretical physicists be doing if they got interested in this field?
  • In the interaction between physics and biology, what happens to chemistry?
  • How much biology do I need to know to make progress?
  • Why do physicists and biologists seem to be speaking such different languages?
  • Can I be interested in biological problems and still be a physicist, or do I have to become a biologist?"
The entire introduction (indeed, the entire book) is an attempt to answer these questions. If you don't have access to the book, you could read his similarly themed article available on the physics archive: Perspectives on Theory at the Interface of Physics and Biology.

Like IPMB, Bialek's book has many homework problems. Readers of this blog know that I enjoy reducing biological and medical physics principles down to homework problems, so this footnote from Bialek's introduction resonated with me:
"In some sections I found it difficult to formulate manageable problems. I worry that this reflects poorly on my understanding."
For those of you who prefer video over text, below is a three-part interview with Bialek from the International Centre for Theoretical Physics




Also, here is a video of Bialek giving the 2017 Buhl Lecture "The Physics of Life: How Much Can We Calculate?"


Enjoy!

Friday, November 10, 2017

Facebook

The Intermediate Physics for Medicine and Biology Facebook Group has now reached 150 members.

Yes, IPMB has a Facebook group. I use it to circulate blog posts every Friday morning, but I occasionally share other posts of interest to readers of IPMB. The group photo is my Ideal Bookshelf picture highlighting books about physics applied to medicine and biology.

Group members include my family (including my dog Suki Roth, who has her own Facebook Page) and former students. But members I don't know come from countries all over the world, including:
In particular, many members are from India and Pakistan.

I am amazed and delighted to have members from all over the world. I don't know if universities teach classes based on IPMB in all these places, or if people just stumble upon the group.

The IPMB Facebook group welcomes everyone interested in physics applied to medicine and biology. I am delighted to have you. And for those who are not yet members, just go to Facebook, search for "Intermediate Physics for Medicine and Biology," and click "Join Group." Let's push for 200 members!

Friday, November 3, 2017

Countercurrent Transport of Oxygen in the Gills of a Fish

In Section 5.8 of Intermediate Physics for Medicine and Biology, Russ Hobbie and I discuss countercurrent transport.
“The countercurrent principle is found in the renal tubules (Hall 2011, p. 309; Patton et al. 1989, p. 1081), in the villi of the small intestine (Patton et al., 1989, p. 915), and in the lamellae of fish gills (Schmidt-Nielsen 1971, p. 45). The principle is also used to conserve heat in the extremities—such as people’s arms and legs, whale flippers, or the leg of a duck. If a vein returning from an extremity runs closely parallel to the artery feeding the extremity, the blood in the artery will be cooled and the blood in the vein warmed. As a result, the temperature of the extremity will be lower and the heat loss to the surroundings will be reduced.”
In a homework problem, Russ and I ask the student to analyze a countercurrent heat exchanger. In this blog post, I present a new exercise studying countercurrent oxygen exchange in fish gills.
Problem 19 ½. Fish use countercurrent transport to increase uptake of oxygen in their gills. Consider a capillary extending from x = 0 to x = L, with blood flowing in the positive x direction. Blood entering the capillary has a low oxygen concentration that we take as zero. Seawater flowing outside the capillary has an oxygen concentration of [O2] where it enters the gill. Consider the case when |ain|=|aout|=a in Eq. 5.24. The goal is to calculate the oxygen concentration in the blood, Cin, and the oxygen concentration in the seawater, Cout, as functions of x, and in particular to determine the blood oxygen concentration at the end of the gill where it reenters the fish's body, Cin(L).

a) Consider the case when the seawater flows in the same direction as the blood. Draw a picture illustrating this case. Derive expressions for Cin(L) and Cout(x) in terms of [O2], a, and L. Plot qualitatively Cin(x) and Cout(x) versus x when aL is greater than 1. 

b) Now consider the case of countercurrent transport, when the seawater flows in the opposite direction as the blood. Draw a picture, derive expressions, and make plots.

c) Which case results in the highest oxygen concentration in the blood when it leaves the gill and enters the fish’s body?

d) Explain why countercurrent transport is so effective using words instead of equations.
The solution is given at the bottom of this blog post. You can probably guess that countercurrent transport is more efficient for absorbing oxygen. In one of my favorite books, How Animals Work, Knut Schmidt-Nielsen describes countercurrent transport in the fish gill. His description would be an excellent answer to part d) of the new homework problem.
“In the lamellae of the fish gill, water and blood flow in opposite directions (figure 27). As a consequence, the blood, just as it is about to leave the gill, encounters the incoming water which still has all its oxygen; that is, the oxygen tension of the blood will approach that of the water before any oxygen has been removed. At the other end of the lamellae, the water that is about to exit encounters venous blood, so that, even though much oxygen has already been removed from the water, more can still be taken from it by the blood. As a result of this arrangement, fish may extract as much as 80 to 90% of the oxygen in the water, an efficiency which could not easily be achieved without a countercurrent flow.”

Friday, October 27, 2017

Twitter

A few months ago I started an account on Twitter. It is not a personal account, but instead is for the Oakland University Center for Biomedical Research, which I direct. If you are interested, the Twitter handle is @OaklandUniv_CBR. Most of my tweets are useful for faculty and students at OU: announcing seminars, congratulating principal investigators for their new grants, highlighting accomplishments of students, and that sort of thing. However, I follow a lot of accounts that are related to Intermediate Physics for Medicine and Biology. If you are on Twitter, you might like these:
If you use Twitter and know some accounts that readers of IPMB should follow, mention them in the comments.

Enjoy!

Friday, October 20, 2017

Galvani’s Spark: The Story of the Nerve Impulse

I recently finished a wonderful history of neurophysiology. Galvani’s Spark: The Story of the Nerve Impulse, by Alan McComas, covers several topics that Russ Hobbie and I discuss in Intermediate Physics for Medicine and Biology, such as the nerve action potential, patch clamping, and the structure of the potassium channel. While I enjoyed these parts of the book, I particularly liked the earlier history about scientists like Charles Sherrington and Edgar Adrian.

One of my favorite chapters is about Keith Lucas, an English physiologist who worked at Cambridge. Lucas showed that when he increased the strength of an electrical stimulus to a muscle, the response increased in discrete steps. From this, he deduced that each fiber responded in an all-or-none way, and the increase in response with stimulus strength resulted from recruiting more fibers. Lucas had the skills of an engineer as well as a biologist, and would make his own equipment to record action potentials. He probably would have made many more discoveries, except that during World War I he left academic research to work for the military. McComas describes the work well.
"Living in a small wooden hut and rising a 4 in the morning, Lucas grappled with a number of problems that beset the pilots of the early flying machines. One was to improve a bombsight, and another to eliminate the unreliability of the pilot’s compass as the plane was made to turn. Once again, just as it had in the Physiological Laboratory in Cambridge, Lucas’s flair for analysis and design, and for constructing equipment himself, served him in good stead, and the problems were solved. To gain first-hand experience of a particular problem, and to see if his solution was effective, Lucas would fly himself, initially as a passenger and then as a trained pilot. For this, he transferred to the Central Flying School at Upavon in Wiltshire."
Tragically, in October 1916 he was killed in a midair collision between two planes.

Another interesting chapter was about three American neurophysiologists—Joseph Erlanger, Herbert Gasser, and George Bishop—who pioneered the use of an oscilloscope for recording action potentials. Gasser is portrayed as saintly, but Erlanger doesn’t come across as an attractive figure. At one point, Bishop published a paper without passing it by Erlanger first, and Erlanger threw a fit.
"Erlanger’s violent temper was well known to this family, but at work it had usually been controlled. Now, however, it was unleashed in its full fury. Bishop was sent for, an accusatory letter written, and then came expulsion from the physiology department."
In 1944, Erlanger and Gasser were awarded the Nobel Prize in Physiology or Medicine, but Bishop was not included. McComas disagrees with this decision, describing Bishop as “the man who should have shared the Nobel Prize with Gasser and Erlanger.”

Another interesting story is of the debate between Henry Dale and John Eccles about the nature of the nerve-muscle synapse. Dale favored a chemical synapse, with acetylcholine as the neurotransmitter. Eccles championed a synapse having a direct electrical connection. Apparently they engaged in a heated battle at the 1935 Cambridge meeting of the Physiological Society. Dale won this battle, and shared the 1936 Nobel Prize with Otto Loewi for their “discoveries relating to chemical transmission of nerve impulses”. Eccles, after a difficult time finding his scientific home, eventually made landmark discoveries about neural transmission in the central nervous system, and won his own Nobel Prize.

American Kenneth Cole is a complex character. On the one hand, Cole was generous in sharing his ideas with the young Alan Hodgkin when Hodgkin visited his Woods Hole laboratory in 1938. Yet, McComas writes
"Respected and admired as a pioneer in the study of the nerve impulse, the recipient of medals and honorary degrees, Kenneth Cole was not content. This kind and unassuming man continued to resent the fact that his preparation and his voltage clamp had been used by Hodgkin without due acknowledgement."
He adds this interesting insight: “Unlike Cole, perpetually bedeviled by problems of one kind or another, success always seemed to follow Hodgkin.”

Another scientist depicted almost tragically is the Spaniard Rafael Lorente de No. McComas says
"The publication of the Hodgkin-Huxley papers had been a bitter blow. Having labored for 10 years on his monumental study of peripheral nerve, Lorente now found that it was largely irrelevant, or, even worse, wrong in its main conclusions….Yet he refused to capitulate, let alone to walk away from a battle that only he wished to right. He would appear at international meetings, rejecting the general applicability of the Hodgkin-Huxley findings, and referring dismissively to the ‘so-called sodium hypothesis.’ It was a sad end to a career that had been so full of promise."
The climax of the book is the story of Alan Hodgkin and Andrew Huxley developing their model of the squid giant axon, a model described in Chapter 6 of IPMB. Here is my favorite passage:
"It was the intention of Hodgkin and Huxley to use the Cambridge University computer—the only computer in the entire university—to carry out the formidable amount of calculation involved, but the machine was undergoing major modifications at the time and would not be available for six months. Huxley then suggested to Hodgkin that he, Huxley, attempt to solve them himself, with the aid of his hand-operated Brunsviga calculating machine. It was an extraordinarily ambitious undertaking. The calculator, rather like an old-fashioned cash register, required that the data were entered by moving small levers in slots to appropriate positions beside numerically inscribed wheels. A handle at the side of the machine would then be turned so many times in one direction or another, and the results read off on the numbered wheels. These results would then have to be written down on paper, before proceeding to the next stage of the calculation. And these steps had to be repeated over and over again. The reconstruction of the action potential required numerical integration, and a complete set of data had to be produced for each small time interval. To calculate a complete ‘run’ required 8 hours of intense mental and physical activity. It has been said that, in all the calculations, more than a million separate steps were involved. It is doubtful if anyone other than Huxley could have brought it off."
If you are looking for a history of the early years of neuroscience, I highly recommend Galvani’s Spark. To tell you the truth, when I started the book I didn’t think it would be this good. Enjoy!

Friday, October 13, 2017

John Clark, Biomedical Engineer (1936-2017)

John W. Clark passed away on August 6, in Houston, Texas. He was a professor of Engineering at Rice University for 49 years.

When I was a graduate student at Vanderbilt University in the 1980s, I was influenced by the papers of Robert Plonsey and his graduate student Clark. They calculated the extracellular electrical potential outside a nerve axon from the transmembrane action potential by expressing the transmembrane potential in terms of its Fourier transform, and then using Bessel functions to calculate the Fourier transform of the extracellular potential. Russ Hobbie and I outline this technique in Problem 30 of Chapter 7 in Intermediate Physics for Medicine and Biology. James Woolsey, my PhD advisor John Wikswo, and I used a similar method—inspired by Clark and Plonsey’s work—to calculate the magnetic field of a nerve axon (see Problem 16 of Chapter 8 in IPMB). Moreover, my first work on the bidomain model of the heart was analyzing cylindrical strands of cardiac tissue using methods that were an extension of Clark and Plonsey’s work. If I were to list the articles that had the biggest impact on my own work, near the top of that list would be Clark and Plonsey’s 1968 paper in the Biophysical Journal (Volume 8, Pages 842-864).

Clark graduated from Case Western Reserve University at about the time this Biophysical Journal  paper was published, and joined the faculty at Rice. Rarely do you see a professor's career span half a century at one institution. He was a Life Fellow of the Institute of Electrical and Electronics Engineers (IEEE) "for contributions to modeling in electrophysiology, and cardiopulmonary systems." He played a role in establishing the field of biomedical engineering, and served as President of the IEEE Engineering in Medicine and Biology Society.

To learn more about Clark and his contributions, see obituaries here, here and here.